stem cells in their environment

team >

simon broad




Centre Manager


Plymouth University, Plymouth, Devon BSc (Hons)Biological Sciences, 1975

research project title

'Analysis of primary human keratinocytes from healthy and diseased tissue'

core expertise

•  Cell culture

•  Viral vectors


[Full PubMed list]

•  'Efficient differentiation of embryonic stem cells into mesodermal precursors by BMP, retinoic acid and Notch signalling.' Torres J, Prieto J, Durupt FC, Broad S, Watt FM. PLoS One. 2012;7(4):e36405. Epub 2012 Apr 30. PMID: 22558462 [PubMed]

•  'Force generation and protease gene expression in organotypic co-cultures of fibroblasts and keratinocytes.' Wall IB, Bhadal N, Broad S, Whawell SA, Mudera V, Lewis MP. J Tissue Eng Regen Med. 2009 Dec;3(8):647-50. PMID: 19701934 [PubMed]

•  'Cultivation and Retroviral Infection of Human Epidermal Keratinocytes.' Watt FM, Broad S, Prowse DM. Cell Biology, A Laboratory Handbook. 2007 Third edition. Volume 1. Chapter 16. Celis JE ed. Academic Press.

•  'The effect of mechanical strain on protease production by keratinocytes'.' Bhadal N, Wall IB, Porter SR, Broad S, Lindahl GE, Whawell S, Lewis MP. Br J Dermatol. 2008 Feb;158(2):396-8. Epub 2007 Dec 7 PMID: 18067479 [PubMed]

•  'Human ovarian surface epithelial cells immortalized with hTERT maintain functional pRb and p53 expression.' Li NF, Broad S, Lu YJ, Yang JS, Watson R, Hagemann T, Wilbanks G, Jacobs I, Balkwill F, Dafou D, Gayther SA. The first two authors (N. F. Li, S. Broad) contributed equally to this paper. Cell Prolif. 2007 Oct;40(5):780-94. PMID: 17877616 [PubMed]

•  'Slug regulates integrin expression and cell proliferation in human epidermal keratinocytes.' Turner FE, Broad S, Khanim FL, Jeanes A, Talma S, Hughes S, Tselepis C, Hotchin NA. J Biol Chem. 2006 Jul 28;281(30):21321-31. Epub 2006 May 17. PMID: 16707493 [PubMed]

•  'Suprabasal alpha 5 beta1 integrin expression stimulates formation of epidermal squamous cell carcinomas without disrupting TGFbeta signaling or inducing spindle cell tumors.' Owens DM, Broad S, Yan X, Benitah SA, Watt FM. Mol Carcinog. 2005 Sep;44(1):60-6. PMID: 15924349 [PubMed]

•  'Keratinocyte differentiation is regulated by the Rho and ROCK signaling pathway.' McMullan R, Lax S, Robertson VH, Radford DJ, Broad S, Watt FM, Rowles A, Croft DR, Olson MF, Hotchin NA. Curr Biol. 2003 Dec 16;13(24):2185-9. PMID: 14680635 [PubMed]

•  'Role of melanoma chondroitin sulphate proteoglycan in patterning stem cells in human interfollicular epidermis.' Legg J, Jensen UB, Broad S, Leigh I, Watt FM. Development. 2003 Dec;130(24):6049-63. Epub 2003 Oct 22. PMID: 14573520 [PubMed]

•  'Cell-based delivery of cytokines allows for the differentiation of a doxycycline inducible oligodendrocyte precursor cell line in vitro.' Muth H, Elmshauser C, Broad S, Schipke C, Kettenmann H, Beck E, Kann M, Motta I, Chen U. J Gene Med. 2001 Nov-Dec;3(6):585-98. PMID: 11778905 [PubMed]

•  'Subchromosomal positioning of the epidermal differentiation complex (EDC) in keratinocyte and lymphoblast interphase nucle.'. Williams RR, Broad S, Sheer D, Ragoussis J. Exp Cell Res. 2002 Jan 15;272(2):163-75. PMID: 11777341 [PubMed]

•  'A role for mitogen-activated protein kinase activation by integrins in the pathogenesis of psoriasis.' Haase I, Hobbs RM, Romero MR, Broad S, Watt FM. J Clin Invest. 2001 Aug;108(4):527-36. PMID: 11518726 [PubMed]

•  'beta1 integrins regulate keratinocyte adhesion and differentiation by distinct mechanisms.' Levy L, Broad S, Diekmann D, Evans RD, Watt FM. Mol Biol Cell. 2000 Feb;11(2):453-66. PMID: 10679006 [PubMed]

•  'Optimised retroviral infection of human epidermal keratinocytes: long-term expression of transduced integrin gene following grafting on to SCID mice.' Levy L, Broad S, Zhu AJ, Carroll JM, Khazaal I, Péault B, Watt FM. Gene Ther. 1998 Jul;5(7):913-22. PMID: 9813662 [PubMed]

•  'The bombesin/GRP receptor transfected into Rat-1 fibroblasts couples to phospholipase C activation, tyrosine phosphorylation of p125FAK and paxillin and cell proliferation.' Charlesworth A, Broad S, Rozengurt E. Oncogene. 1996 Mar 21;12(6):1337-45. PMID: 8649836 [PubMed]

•  'Mitogenic signaling by transfected neuromedin B receptors in Rat-1 cells.' Lach EB, Broad S, Rozengurt E. Cell Growth Differ. 1995 Nov;6(11):1427-35. PMID: 8562481 [PubMed]

•  'The human CCKB/gastrin receptor transfected into rat1 fibroblasts mediates activation of MAP kinase, p74raf-1 kinase, and mitogenesis.' Seufferlein T, Withers DJ, Broad S, Herget T, Walsh JH, Rozengurt E. Cell Growth Differ. 1995 Apr;6(4):383-93. PMID: 7794806 [PubMed]

•  'Overexpression of the myristoylated alanine-rich C-kinase substrate in Rat1 cells increases sensitivity to calmodulin antagonists.' Herget T, Broad S, Rozengurt E. Eur J Biochem. 1994 Oct 15;225(2):549-56. PMID: 7957169 [PubMed]

•  'Expression of the major protein kinase C substrate, the acidic 80-kilodalton myristoylated alanine-rich C kinase substrate, increases sharply when Swiss 3T3 cells move out of cycle and enter G0.' Herget T, Brooks SF, Broad S, Rozengurt E. Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2945-9. PMID: 8464911 [PubMed]

•  'Whole-cell recording of neuroblastoma x glioma cells during downregulation of a major substrate, 80K/MARCKS, of protein kinase C'. Civan MM, Robbins J, Broad S, Rozengurt E, Brown DA. J Membr Biol. 1993 Apr;133(1):51-9. PMID: 8320719 [PubMed]

•  'Immunoprecipitation of viral polypeptides of equid herpesvirus 1 and 4 by serum from experimentally infected ponies'. Ahmed SM, Broad S, Edington N. Vet Microbiol. 1993 Mar;34(3):205-19. PMID: 8384737 [PubMed]

•  'Relationship between the major protein kinase C substrates acidic 80-kDa protein-kinase-C substrate (80K) and myristoylated alanine-rich C-kinase substrate (MARCKS). Members of a gene family or equivalent genes in different species'. Herget T, Brooks SF, Broad S, Rozengurt E. Eur J Biochem. 1992 Oct 1;209(1):7-14. PMID: 1396720 [PubMed]

'The expression of 80K/MARCKS, a major substrate of protein kinase C (PKC), is down-regulated through both PKC-dependent and -independent pathways. Effects of bombesin, platelet-derived growth factor, and cAMP.' Brooks SF, Herget T, Broad SC, Rozengurt E. J Biol Chem. 1992 Jul 15;267(20):14212-8. PMID: 1378435 [PubMed]

•  'Superinfection with porcine cytomegalovirus initiating transplacental infection.' Edington N, Broad S, Wrathall AE, Done JT. Vet Microbiol. 1988 Feb;16(2):189-93. PMID: 2836992 [PubMed]

•  'Rapid diagnosis and characterization of equid herpesvirus 1 using monoclonal antibodies.' Edington N, Bridges CG, Broad SC. Tierarztl Prax Suppl. 1987;2:37-40. PMID: 2438808 [PubMed]